Cystine Lactose Electrolyte Deficient (CLED) Agar is used for the differentiation and enumeration of microorganisms in urine. A medium for urine culture where the absence of electrolytes inhibits the swarming of Proteus spp. Cystine is added for the benefit of those organisms which have a specific cystine requirement. Differentiation of lactose and non-lactose fermenters is achieved using bromothymol blue as pH indicator. This medium supports the growth of Streptococcus pyogenes and most other fastidious organisms that do not require blood.
- Analyte: coliforms, E. coli, enterobacteriaceae, enterococci, pseudomonas, staphylococcus, streptococci
- Platform: dehydrated culture media
- Formula: balanced peptone 4.0g/L, beef extract 3.0g/L, tryptone 4.0g/L, lactose 10.0g/L, l-cystine 0.128g/L, bromothymol blue 0.02g/L, agar 15.0g/L
- Grams per litre: 36.00
- Litres per 500g: 14.00
- Plates per 500gL 760.00
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